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Laetitia Nikles
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Nikles
Laetitia
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{"id":7856918,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":{"translations":{"fr":{"id":9451,"title":"Swiss Centre Human Applied Toxicology.\r\n","description":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n"},"en":{"id":9452,"title":"Swiss Centre Human Applied Toxicology.\r\n","description":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n"},"de":{"id":9453,"title":"Swiss Centre Human Applied Toxicology.\r\n","description":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n"}},"id":53966,"acronym":"SCAHT-6","mainTitle":"Swiss Centre Human Applied Toxicology.\r\n","mainDescription":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n","value":"168500.00","finished":true,"pilier":6,"url":null,"keywords":null,"disciplines":[],"axes":[],"partners":[],"collaborators":[{"id":7856917,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"dominiqu.trinchan","project":53966},{"id":7856918,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":53966},{"id":7856919,"role":"ME","display":true,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"adrien.roux","project":53966},{"id":7856920,"role":"RP","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"luc.stoppini","project":53966}],"dataHub":true,"startAt":"2016-01-01T00:00:00+01:00","endAt":"2016-12-31T00:00:00+01:00","fundingSource":"SCAHT; hepia inSTI; hepia inSTI","publications":[],"projectUrl":null,"repo_name":null}}{"id":7786838,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":{"translations":{"fr":{"id":7597,"title":"In vitro models for toxicity assessment\r\n","description":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \r\nGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, and"},"en":{"id":7598,"title":"In vitro models for toxicity assessment\r\n","description":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \r\nGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, and"},"de":{"id":7599,"title":"In vitro models for toxicity assessment\r\n","description":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \r\nGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, and"}},"id":38824,"acronym":"SCAHT4_2014","mainTitle":"In vitro models for toxicity assessment\r\n","mainDescription":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \r\nGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, and","value":"340000.00","finished":true,"pilier":6,"url":null,"keywords":null,"disciplines":[],"axes":[],"partners":[],"collaborators":[{"id":7786837,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"dominiqu.trinchan","project":38824},{"id":7786838,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":38824},{"id":7786839,"role":"ME","display":true,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"adrien.roux","project":38824},{"id":7786840,"role":"RP","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"luc.stoppini","project":38824}],"dataHub":true,"startAt":"2014-04-01T00:00:00+02:00","endAt":"2014-12-31T00:00:00+01:00","fundingSource":"UNIGE; hepia inSTI; hepia inSTI","publications":[],"projectUrl":null,"repo_name":null}}{"id":7786582,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":{"translations":{"fr":{"id":7042,"title":"In vitro models for toxicity assessement.","description":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\n\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \rGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, an"},"en":{"id":7043,"title":"In vitro models for toxicity assessement.","description":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\n\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \rGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, an"},"de":{"id":7044,"title":"In vitro models for toxicity assessement.","description":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\n\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \rGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, an"}},"id":36062,"acronym":"SCAHT 3","mainTitle":"In vitro models for toxicity assessement.","mainDescription":"\"3 Neurotoxicology in vitro\r\n3.2 Human in vitro 2D and 3D models of mature neural networks for Neurotoxicity assessment\r\n\r\nProject Leader: Luc Stoppini\r\n Partners: F. Tschudi-Monnet; C. Degeyter; Alex Scherl\r\nFinal histological characterization of 2D and 3 D human neural networks: \r\nWe have generated human neural cells and tissue derived from hESCs. We will perform the final characterization of the different 2D and 3 D neural cultures.\r\nImmunostainings of the different neural makers to assess the presence and the organization of the different cells types (GFAP: for astrocytes; CNPase and MBP for oligodendrocytes; MAP2, NF,NeuN for neurones). We will also perform some electron microscopy studies to assess the precise morphology of the differentiated nervous tissues.\r\nDeliverable: Report on the histological characterization 01.01.2013\r\n31.05.2013\r\nFunctional Characterization of the neural networks generated from hESCs by electrophysiological studies: \r\nElectrophysiological recordings will be performed using neural tissues generated from hESCs and laid down onto MEAs. Spontaneous as well as evoked field potentials will be recorded in control nervous tissues and after exposure of the neuro-glial networks to reference pharmacological molecules to verify that the nervous tissues are responding similarly to primary neural tissues.\r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization. 01.01.2013\r\n31.06.2013\r\nAcute neurotoxicity studies: \r\nAcute dose-responses of neurotoxicants will be performed in 3D human neural networks to assess their effects on the neuronal activity in vitro by means of electrophysiological M.E.A. recordings. \r\nDeliverable: Report on the 3D neural tissue electrophysiological characterization for reference neurotoxic compounds. 01.07.2013\r\n31.12.2013\r\nGene expression profile in 3D neural tissues: \rGene expression profile: We will verify gene expression toxicity signatures using different types of xenobiotics (control and known to induce adverse effects on neural tissues). Human neuro-glial networks will be treated acutely or repeatedly with different concentrations of a series of different types of neurotoxicants. mRNA will be isolated at different culture time points and measured by RT-qPCR. \r\nDeliverable: Report on the 3D neural tissue gene profile of specific markers in control and using reference neurotoxic molecules. 01.04.2013\r\n31.12.2013\r\nProteomic analyses of 3D human neural tissues: \r\nThe proteomic analysis profile will be performed in control conditions and after treatment of 3 D human neural tissues with reference neurotoxic molecules.\r\nDeliverable: Report on proteomic neurotoxic profile of 3D human neural tissues. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using calcium mobilization assay: \r\nMeasurement of calcium mobilization: Variations in the concentration of intracellular calcium, which is known to trigger a number of events including the release of synaptic transmitter, will be measured using Fluo-4 Flexstation Calcium assay kit. Human neuronal cells will be grown in 96 well plates to fit to the workflow system. Reference toxic molecules will be tested to validate the approach.\r\nDeliverable: Report on the validation of the Calcium mobilization as a functional assay to assess neurotoxicity. 01.03.2013\r\n31.12.2013\r\nFunctional activities of 2D neural networks using Neurotransmitter Transporter Uptake Assay: \r\nHomogeneous Neurotransmitter Transporter Uptake Assay: The assay includes a fluorescent indicator dye that mimics the neurotransmitters serotonin, norepinephrine, and dopamine which are actively transported into the cells via the specific neurotransmitter transporters. The fluorescent substrate that mimics the biogenic amine neurotransmitters is then taken up into the cell through those specific transporters, resulting in increased intracellular fluorescence intensity. This homogeneous, fluorescent assay is robust, sensitive, and specific, an","value":"340000.00","finished":true,"pilier":6,"url":null,"keywords":null,"disciplines":[],"axes":[],"partners":[],"collaborators":[{"id":7786581,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"dominiqu.trinchan","project":36062},{"id":7786582,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":36062},{"id":7786583,"role":"ME","display":true,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"adrien.roux","project":36062},{"id":7786584,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"herve.sthioul","project":36062},{"id":7786585,"role":"RP","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"luc.stoppini","project":36062}],"dataHub":true,"startAt":"2013-01-01T00:00:00+01:00","endAt":"2014-06-30T00:00:00+02:00","fundingSource":"hepia inSTI; Universit\u00e9 de Gen\u00e8ve; hepia inSTI","publications":[],"projectUrl":null,"repo_name":null}}{"id":7786199,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":{"translations":{"fr":{"id":6415,"title":"NeuroSpectrum\r\n","description":"The central nervous system (CNS) is a very complicated system with hundreds of different active molecules involved in numerous processes in different anatomical and functional parts of the brain. The time course of synaptic currents is one of the main factors determining how a single neuron integrates information coming from different inputs. Establishing the temporal and concentration profiles of neurotransmitters during synaptic release is an essential step to monitor inter-neuronal communications in the central nervous system in normal or pathological conditions. We propose to develop a new in vitro assay that will allow us, by measuring the levels of neurotransmitters released from human neural networks within a 3D neural model developed and produced by Neurix (MiniBrain), to assess the beneficial or deleterious effects of new therapeutically compounds or chemicals found in our food and our environment. The profiling of neurotransmitters released from human neural tissues will thus represent a more predictive and cost effective tool that will be integrated into pharmaceutical and chemical industry platforms for early safety and neurotoxicology assays. The aim of this project is to deliver a functional in vitro assay for neurotoxicity assessment which will be commercialized by Neurix. \r\n"},"en":{"id":6416,"title":"NeuroSpectrum\r\n","description":"The central nervous system (CNS) is a very complicated system with hundreds of different active molecules involved in numerous processes in different anatomical and functional parts of the brain. The time course of synaptic currents is one of the main factors determining how a single neuron integrates information coming from different inputs. Establishing the temporal and concentration profiles of neurotransmitters during synaptic release is an essential step to monitor inter-neuronal communications in the central nervous system in normal or pathological conditions. We propose to develop a new in vitro assay that will allow us, by measuring the levels of neurotransmitters released from human neural networks within a 3D neural model developed and produced by Neurix (MiniBrain), to assess the beneficial or deleterious effects of new therapeutically compounds or chemicals found in our food and our environment. The profiling of neurotransmitters released from human neural tissues will thus represent a more predictive and cost effective tool that will be integrated into pharmaceutical and chemical industry platforms for early safety and neurotoxicology assays. The aim of this project is to deliver a functional in vitro assay for neurotoxicity assessment which will be commercialized by Neurix. \r\n"},"de":{"id":6417,"title":"NeuroSpectrum\r\n","description":"The central nervous system (CNS) is a very complicated system with hundreds of different active molecules involved in numerous processes in different anatomical and functional parts of the brain. The time course of synaptic currents is one of the main factors determining how a single neuron integrates information coming from different inputs. Establishing the temporal and concentration profiles of neurotransmitters during synaptic release is an essential step to monitor inter-neuronal communications in the central nervous system in normal or pathological conditions. We propose to develop a new in vitro assay that will allow us, by measuring the levels of neurotransmitters released from human neural networks within a 3D neural model developed and produced by Neurix (MiniBrain), to assess the beneficial or deleterious effects of new therapeutically compounds or chemicals found in our food and our environment. The profiling of neurotransmitters released from human neural tissues will thus represent a more predictive and cost effective tool that will be integrated into pharmaceutical and chemical industry platforms for early safety and neurotoxicology assays. The aim of this project is to deliver a functional in vitro assay for neurotoxicity assessment which will be commercialized by Neurix. \r\n"}},"id":31942,"acronym":"NeuroSpectrum","mainTitle":"NeuroSpectrum\r\n","mainDescription":"The central nervous system (CNS) is a very complicated system with hundreds of different active molecules involved in numerous processes in different anatomical and functional parts of the brain. The time course of synaptic currents is one of the main factors determining how a single neuron integrates information coming from different inputs. Establishing the temporal and concentration profiles of neurotransmitters during synaptic release is an essential step to monitor inter-neuronal communications in the central nervous system in normal or pathological conditions. We propose to develop a new in vitro assay that will allow us, by measuring the levels of neurotransmitters released from human neural networks within a 3D neural model developed and produced by Neurix (MiniBrain), to assess the beneficial or deleterious effects of new therapeutically compounds or chemicals found in our food and our environment. The profiling of neurotransmitters released from human neural tissues will thus represent a more predictive and cost effective tool that will be integrated into pharmaceutical and chemical industry platforms for early safety and neurotoxicology assays. The aim of this project is to deliver a functional in vitro assay for neurotoxicity assessment which will be commercialized by Neurix. \r\n","value":"579104.00","finished":true,"pilier":6,"url":null,"keywords":null,"disciplines":[],"axes":[],"partners":[{"id":1842619,"name":"","confidential":false,"types":[{"id":3,"code":"RP"},{"id":4,"code":"CO"}],"institution":"hepia inSTI","class":"academique"},{"id":1842620,"name":"","confidential":false,"types":[{"id":4,"code":"CO"}],"institution":"Universit\u00e9 de Gen\u00e8ve","class":"academique"},{"id":1842621,"name":null,"confidential":false,"types":[{"id":2,"code":"TER"},{"id":5,"code":"TERRAIN"}],"institution":"Neurix","class":"professionnel"},{"id":1842622,"name":"","confidential":false,"types":[{"id":4,"code":"CO"}],"institution":"Universit\u00e9 de Gen\u00e8ve, Pharma","class":"academique"},{"id":1842623,"name":"Stoppini Luc","confidential":false,"types":[{"id":3,"code":"RP"},{"id":4,"code":"CO"}],"institution":"hepia inSTI","class":"academique"}],"collaborators":[{"id":7786199,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":31942},{"id":7786200,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"sandrine.rutz","project":31942},{"id":7786201,"role":"RP","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"luc.stoppini","project":31942}],"dataHub":true,"startAt":"2012-01-01T00:00:00+01:00","endAt":"2014-04-30T00:00:00+02:00","fundingSource":"CTI","publications":[],"projectUrl":null,"repo_name":null}}{"id":7764633,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":{"translations":{"fr":{"id":9250,"title":"Swiss Centre Human Applied Toxicology.\r\n","description":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n"},"en":{"id":9251,"title":"Swiss Centre Human Applied Toxicology.\r\n","description":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n"},"de":{"id":9252,"title":"Swiss Centre Human Applied Toxicology.\r\n","description":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n"}},"id":48805,"acronym":"SCAHT-5","mainTitle":"Swiss Centre Human Applied Toxicology.\r\n","mainDescription":"Human in vitro 2D and 3D models of mature neural networks for neurotoxicity assessment.\r\n","value":"168095.23","finished":true,"pilier":6,"url":null,"keywords":null,"disciplines":[],"axes":[],"partners":[],"collaborators":[{"id":7764632,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"dominiqu.trinchan","project":48805},{"id":7764633,"role":"ME","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"laetitia.nikles","project":48805},{"id":7764634,"role":"ME","display":true,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"adrien.roux","project":48805},{"id":7764635,"role":"RP","display":false,"displayRole":true,"displayFinancialPartner":true,"displayAcademicPartner":true,"displayProfessionalPartner":true,"collaborator":"luc.stoppini","project":48805}],"dataHub":true,"startAt":"2015-01-01T00:00:00+01:00","endAt":"2015-12-31T00:00:00+01:00","fundingSource":"SCAHT; 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